Tirzepatide Reconstitution Calculator
Select your vial size and target concentration to instantly calculate the exact volume of bacteriostatic water to add — plus a complete U-100 insulin syringe dose reference table.
Reconstitution Calculator
Select your vial size and target concentration — BAC water volume and dose table calculated instantly.
BAC Water to Add
6mL
15 mg ÷ 2.5 mg/mL
Resulting Concentration
2.5mg/mL
15 mg total peptide
Total Solution Volume
6mL
All peptide dissolved in BAC water
Dose Reference Table
U-100 insulin syringe · 1 unit = 0.01 mL · Concentration: 2.5 mg/mL
| Research Dose | Units on Syringe(U-100) | Volume |
|---|---|---|
0.5 mg | 20units | 0.200mL |
1 mg | 40units | 0.400mL |
2.5 mgCommon | 100units | 1.000mL |
5 mgCommon | 200units | 2.000mL |
7.5 mgCommon | 300units | 3.000mL |
10 mgCommon | 400units | 4.000mL |
12.5 mg | 500units | 5.000mL |
15 mg | 600units | 6.000mL |
Formula: Units = (dose ÷ concentration) × 100. Based on U-100 insulin syringe where each unit = 0.01 mL. Grayed-out rows exceed your 15mg vial total.
Research purposes only. This calculator is provided for laboratory research use. Not intended for human administration, clinical use, or medical advice. Researchers are solely responsible for compliance with applicable regulations and safe laboratory practices.
Research Supplies
Ready to begin your tirzepatide research? Source the peptide and reconstitution supplies used in this calculator.
How to Reconstitute Tirzepatide
Step-by-step protocol for safely preparing lyophilized tirzepatide for laboratory research. Follow aseptic technique throughout.
Gather supplies
You'll need: lyophilized tirzepatide vial, bacteriostatic water (BAC water), 1 mL or 3 mL syringe for reconstitution, U-100 insulin syringes for dosing, alcohol wipes, and a clean workspace.
Sanitize all surfaces
Wipe the rubber septum of the tirzepatide vial and the BAC water vial with a fresh 70% isopropyl alcohol wipe. Allow to air dry for 10–15 seconds. Never touch the cleaned septum.
Draw BAC water
Using the reconstitution syringe, draw the calculated volume of BAC water from the vial (use the calculator above to determine the exact mL). Remove any air bubbles by gently tapping the syringe.
Inject along the vial wall — do not agitate
Insert the needle into the lyophilized powder vial at an angle so the BAC water runs slowly down the inside glass wall. Never inject directly onto the powder cake. This prevents denaturation.
Swirl gently — never shake
Gently rotate the vial between your fingers in a slow circular motion. The powder should dissolve within 30–60 seconds into a clear, colorless solution. Do not vortex or shake vigorously.
Verify clarity and label
Hold the vial up to light. The solution should be clear and free of particles or cloudiness. Label the vial immediately with: peptide name, concentration, date reconstituted, and initials.
Store correctly
Refrigerate the reconstituted vial at 2–8°C (standard refrigerator temperature). Do not freeze. Keep away from light. Use within 28 days of reconstitution for best peptide integrity.
Storage Quick Reference
Proper storage is critical to maintaining peptide integrity and research validity.
Lyophilized (dry powder)
Up to 24 months at −20°C
Or 12 months at 2–8°C (refrigerator)
Reconstituted solution
28 days at 2–8°C
Do not freeze after reconstitution
Handling temperature
Room temp ≤ 30 min
Minimize exposure to warmth during prep
Key rules: Never freeze a reconstituted solution — this causes aggregation and loss of activity. Keep all peptides away from direct light and heat. Use amber or opaque storage containers where possible. Label every vial immediately after preparation.