Tirzepatide Research Peptide Reconstitution Guide

Tirzepatide Reconstitution for Research

Tirzepatide is supplied as a lyophilized powder in 15mg, 30mg, and 60mg vials for research purposes. Proper reconstitution ensures peptide integrity and reproducible research results.

Materials

• Tirzepatide vial (15mg, 30mg, or 60mg)
• Bacteriostatic water (0.9% benzyl alcohol preserved)
• Sterile insulin syringe (28–31 gauge)
• Alcohol swabs
• Sterile gloves
• Refrigerator storage

Concentration Calculations

15mg Vial

• Add 3mL BAC water → 5mg/mL (each 0.1mL = 0.5mg)
• Add 5mL BAC water → 3mg/mL (each 0.1mL = 0.3mg)

30mg Vial

• Add 3mL BAC water → 10mg/mL
• Add 6mL BAC water → 5mg/mL

60mg Vial

• Add 6mL BAC water → 10mg/mL
• Add 12mL BAC water → 5mg/mL

Step-by-Step Protocol

Preparation

Allow vial to reach room temperature (20–25°C) before reconstitution. Inspect the lyophilized cake — should be white/off-white, intact, and free from discoloration.

Adding Diluent

1. Wipe vial stopper with alcohol swab, allow 10 seconds to dry
2. Draw calculated BAC water volume into syringe
3. Insert needle into vial at 45° angle
4. Slowly inject water down the side of the vial glass (NOT directly onto the powder)
5. Remove syringe

Dissolving

• Gently swirl or roll vial between palms
• Do NOT vortex or shake
• Allow 2–5 minutes for complete dissolution
• Final solution: clear, colorless to slightly yellow

Storage Conditions

• Lyophilized (unopened): −20°C to −80°C for long-term; 2–8°C for short-term
• Reconstituted: 2–8°C, use within 4–6 weeks
• Protect from light; do not freeze reconstituted solution

Quality Control Checks

• No visible particulate matter
• Clear solution (mild yellow tint acceptable)
• No gel formation or cloudiness
• Vial integrity maintained throughout

For laboratory research only. Not for human administration.